Coding

Part:BBa_K2374004:Design

Designed by: Jianfeng Zhou   Group: iGEM17_Tongji_China   (2017-10-23)


GAL4 (galactose-responsive transcription factor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI.rc site found at 1813
    Illegal SapI site found at 502


Design Notes

site-directed mutagenesis: TCTAGA--TCAAGA(XbaI) CTGCAG--CTGCTG(PstI)


Source

Genomic Sequence: NC_001148.4 (NCBI)

References